Biomedical subjects

Zbigniew Ruszczak

Publications and source records attributed to Zbigniew Ruszczak.

5 recordsLinked to original sources

PubMed2006

Tissue-engineered temporary wound coverings. Important options for the clinician.

Finding an ideal temporary wound dressing is challenging. Although TransCyte, built on Biobrane, offers many of the characteristics of the ideal wound dressing, and may have added benefits from the delivery of extra-cellular matrix (ECM) and growth factors to the wound, the downside of high cost and less convenient storage and usage are considerable barriers to its broader adoption. The low cost and established clinical utility of Biobrane sets the bar high for new products.

Burns↗

PubMed2006

Update on dermal substitutes.

Tissue-engineered biological dressings offer promise in the treatment of burns, chronic ulcers, donor site and other surgical wounds, and a variety of dermatologic conditions. Despite this promise, cellular tissue-engineered products such a Dermagraft and Apligraf have suffered setbacks in recent years with a lower market share than the commercial promoters of these products anticipated. AlloDerm acellular dermal matrix, an older technology than these cell-based products, has made strong progress in winning over clinicians in various disciplines. Similarly, Integra Bilayer Matrix Wound Dressing (BMWD) continues to gain acceptance beyond its original burn audience. A review on the products is offered.

Chondroitin Sulfates↗

PubMed2004

Influence of different collagen species on physico-chemical properties of crosslinked collagen matrices.

Collagen-based scaffolds are appealing products for the repair of cartilage defects using tissue engineering strategies. The present study investigated the species-related differences of collagen scaffolds with and without 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS)-crosslinking. Resistance against collagenase digestion, swelling ratio, amino acid sequence, shrinkage temperature, ultrastructural matrix morphology, crosslinking density and stress-strain characteristics were determined to evaluate the physico-chemical properties of equine- and bovine-collagen-based scaffolds. Three-factor ANOVA analysis revealed a highly significant effect of collagen type (p=0.0001), crosslinking (p=0.0001) and time (p=0.0001) on degradation of the collagen samples by collagenase treatment. Crosslinked equine collagen samples showed a significantly reduced swelling ratio compared to bovine collagen samples (p< 0.0001). The amino acid composition of equine collagen revealed a higher amount of hydroxylysine and lysine. Shrinkage temperatures of non-crosslinked samples showed a significant difference between equine (60 degrees C) and bovine collagen (57 degrees C). Three-factor ANOVA analysis revealed a highly significant effect of collagen type (p=0.0001), crosslinking (p=0.0001) and matrix condition (p=0.0001) on rupture strength measured by stress-strain analysis. The ultrastructure, the crosslinking density and the strain at rupture between collagen matrices of both species showed no significant differences. For tissue engineering purposes, the higher enzymatic stability, the higher form stability, as well as the lower risk of transmissible disease make the case for considering equine-based collagen. This study also indicates that results obtained for scaffolds based on a certain collagen species may not be transferable to scaffolds based on another, because of the differing physico-chemical properties.

Animals↗

PubMed2003

Effect of collagen matrices on dermal wound healing.

Dermal substitution and wound healing are areas of medicine in which there have been many recent advances, but neither the commercially available products nor the products currently described in experimental studies are able to fully substitute for natural living skin. There is an overall consensus that to heal wounds, the substitution of connective tissue matrix, the main component of each wound, is necessary. Both artificial and natural polymers have been used to reconstitute dermis. Nowadays, collagen has been discovered again. Collagen is a natural substrate for cellular attachment, growth and differentiation, and promotes cellular proliferation and differentiation. Once dermis reconstruction is done, the covering of the wound surface with both in vitro expanded epidermis and autologous split-skin transplants is significantly easier and has an improved chance of success. Nowadays, many commercial and experimental products have been introduced to improve cutaneous wound healing. This review discusses some of both acellular and cell-containing products used in the treatment of skin wounds.

Animals↗

PubMed2003

Collagen as a carrier for on-site delivery of antibacterial drugs.

Due to its biocompatibility and well-established safety profile, collagen represents a favourable matrix for on-site drug delivery. In this review, we summarize some of the recent developments and applications of collagen as a biomaterial in drug delivery systems for antibiotics, especially gentamicin. The main clinical and experimental applications covered include: treatment and prophylaxis of bone and soft tissue infections, wound healing, as well as ophthalmic and periodontal treatment. Advantages of local drug application and the rationale of use local drug delivery systems for adjuvant (ancillary) therapy are discussed. Recent efforts in the use of collagen and collagen-synthetic polymer composites for controlled drug delivery as well as collagen-based diffusion membranes for prolonged drug release have also been included in this review.

Absorbable Implants↗