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Zhi-Lin Wang

Publications and source records attributed to Zhi-Lin Wang.

6 recordsLinked to original sources
PubMed2005

[Significance of dynamic detection of WT1 expression on monitoring minimal residual disease in leukemia patients following allogeneic bone marrow transplantation].

OBJECTIVE: To investigate the significance of monitoring Wilms' tumor gene (WT1) expression level in bone marrow of leukemia patients following allogeneic bone marrow transplantation (allo-BMT). METHODS: Real-time quantitative reverse transcription polymerase chain reaction method was established for measuring WT1 and GAPDH expression levels in bone marrow cells of 15 patients with leukemia, including a total of 111 specimens during the follow-up, and in 23 non-leukemia patients by using LightCycler. Normalized WT1 expression level (WT1(N)) was determined as a ratio of WT1 to GAPDH times 10(4). RESULTS: The median expression levels of WT1(N) in 17 samples of newly diagnosed patients, 6 samples of relapsed patients, 88 samples from leukemia patient in complete remission and 23 samples of non-leukemic controls were 40.18 (5.48 to 510.27), 125.89 (34.50 to 273.95), 4.80 (0 to 56.96) and 1.47 (0 to 8.56) respectively. Nonparameter statistic analysis (Mann-Whitney U test) showed that the WT1(N) expression levels in the newly diagnosed group and relapsed group were statistically higher than in those in the complete remission group and non-leukemic controls (all P < 0.01), without significant differences between the complete remission group and control group (P = 0.692) and between the newly diagnosed group and relapsed group (P = 0.595). In general, the dynamic curves of WT1(N) levels following allo-BMT were consistent with the tendency of changes in expression levels of corresponding fusion genes for minimal residual disease (MRD) monitoring. Spearman Rho correlation analysis revealed that the correlation coefficient between the WT1(N) expression levels and BCR/ABL, AML/ETO, PML/RARalpha and MLL/AF17 fusion genes expression were 0.678 (P = 0.00), 0.677 (P = 0.00), 0.806 (P = 0.00) and 0.553 (P = 0.049) respectively. Three patients relapsed after allo-BMT and one patient relapsed before allo-BMT during the follow-up. A re-increment of WT1(N) expression level during follow-up could be detected 40 to 180 days earlier to hematological relapse. CONCLUSION: The WT1 expression level of leukemia patients following allo-BMT measured by real time RT-PCR can be a useful tool for monitoring MRD and warning the clinical relapse during follow-up.

Adolescent↗
PubMed2005

[In vitro stimulation of specific antileukemia T-cell response by dendritic cells derived from CD14+ acute monocytic leukemia cells].

BACKGROUND & OBJECTIVE: Dendritic cells (DCs) or DC-like cells had been successfully induced in vitro from leukemia cells, which may provide a promising immunotherapeutic protocol for leukemia. This study was designed to investigate the efficiency of in vitro generation of dendritic cells from CD14+ acute myelomonocytic (M4) or monocytic (M5) leukemia cells and their ability of stimulating specific antileukemia T-cell response. METHODS: Bone marrow mononuclear cells (BMMNCs) were isolated from 5 M4/M5 leukemia patients with high CD14 expression, and then divided into 3 groups: adherent leukemia cells, nonadherent blasts, and total unfractioned blasts. CD14 expression of the 3 groups was evaluated by flow cytometry (FCM). When cultured with or without granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-4 (IL-4) and tumor necrosis factor alpha (TNF-alpha) for 7-10 days, monocytic leukemia cell-derived dendritic cells (Mo-LDCs) were identified through morphologic observation and immunophenotype analysis using FCM. The immune function of Mo-LDCs was detected through allogeneic mixed lymphocyte reaction (Allo-MLR) and cytotoxicity assay of antileukemia cytotoxic T lymphocytes (CTLs). The leukemic origin of Mo-LDCs was confirmed by chromosomal karyotype analysis combined with the aberrant expression of myeloid antigens. RESULTS: The amount of CD14+ cells, which could differentiate into CD83+ mature DCs under induction of the cytokine combination, was higher in adherent leukemia cells than in nonadherent blasts and total unfractioned blasts. Regarding each 3 cell groups of the same patient or the unfractioned blasts of various patients, initial CD14 expression was positively related to the yield of CD83+ DCs after induction (r = 0.967, P = 0.007). Mo-LDCs exhibited typical morphology and phenotype as mature DCs, induced potent proliferation of homogeneous T cells in Allo-MLR, stimulated the expansion of leukemia-specific CTLs, and continued to possess the cytogenetic abnormalities of the original leukemia, as well as the aberrant expression of myeloid antigens. CONCLUSIONS: In M4/M5 subtype of AML, CD14+ cells could differentiate into immune-competent Mo-LDCs under the induction of the cytokine combination. CD14 expression level may predict the DCs differentiation ability of monocytic leukemia. Mo-LDCs, which possess the classical phenotype and function of DCs, as well as the abnormal leukemic antigens, may be useful for the immunotherapy of M4/M5 AML.

Adolescent↗
PubMed2004

A study on oxygen insertion in dinuclear silver cryptates.

Two novel silver(I) cryptates are reported in this paper. [Ag(2)(L(1)O)](ClO(4))(2).H(2)O and [Ag(2)L(1)](ClO(4))(2).1.5H(2)O were synthesized by the condensation of tris (3-aminopropyl) amine with m-phthalaldehyde in the presence of silver(i) ion, under aerobic and anaerobic conditions, respectively. (The ligand L(1)O represents the oxygen insertion product of L(1).) Cryptates 1, 2 and their hydrogenated ligand H(12)(L(1)O) 3 and H(12)L(1) 4 (obtained by reduction of the cryptates) were investigated by electrospray mass spectroscopy (ES-MS). 1 and 2 were also decomposed by HCl treatment and their products were separated and identified by HPLC and ES-MS. Our experiments show that cryptate 2 is able to activate dioxygen that results in quantitative aliphatic hydroxylation of L(1) on one of its HC=N bonds. Crystal structure analysis shows an interesting difference between 1 and 2 in that 1 is an oxygenated and 2 is a non-oxygenated cryptate. Up to date, ligand hydroxylation has not been achieved in silver(I) complex-O(2) systems.

Journal Article↗
PubMed2002

A study on Cu2Co2SOD and Co2Co2SOD by voltammetry and thin-layer spectroelectrochemistry.

The electrochemistry of Co(2)Co(2)SOD and Cu(2)Co(2)SOD on a pyrolytic graphite electrode (PGE) without using mediators was investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The reversible and quasi-reversible voltammetric responses were observed for Co(2)Co(2)SOD and Cu(2)Co(2)SOD, respectively. Their formal redox potentials and electron numbers involved in electrode reactions were obtained, and are in agreement with those by spectroelectrochemistry (SEC).

Carbon↗