PubMed Health⌕ Search

Biomedical subjects

Zhi-hui Yang

Publications and source records attributed to Zhi-hui Yang.

3 recordsLinked to original sources
PubMed2006

[Detecting the DNA polymorphism of human complement component C8A by PCR-SSCP analysis].

OBJECTIVE: The aim of this study is to establish a new method for detecting the DNA polymorphism of human complement component C8A. METHODS: Based on the point mutation (C-->A) of C8A gene in exon3,The genotypes of 98 unrelated individuals from Han population in Chengdu were studied by polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP)analysis followed by DNA sequencing. RESULTS: The genotypes of C8A * AA, C8A * BB and C8A * AB were observed and the distribution of the genotypes frequencies of C8A in Chengdu Han population was in accordance with Hardy-Weinberg equilibrium. CONCLUSION: The method of PCR-SSCP is reliable, rapid, simple and cost-effective in detecting the DNA polymorphism of C8A, and it is valuable for further application in population genetic study and forensic science practice.

Adult↗
PubMed2006

[Species identification by multiplex amplifying mtDNA-HV I, HV II and cytb regions].

OBJECTIVE: To evaluate the method for species identification by multiplex amplification of mtDNA-HV I, HV II and cytb regions. METHODS: The bloodstains or muscular tissues of human and 16 kinds of animals were collected. DNA were quantified after extraction. Three pairs of primers were mixed in the same reaction and the PCR amplification products were analyzed by PAGE and silver staining. RESULTS: The presence of three bands (278 bp, 358 bp, 425 bp) indicated the samples were from human, while only one band indicated nonhuman origin. The position of the animal's only band is different from the band (358 bp) of human. CONCLUSION: The method of species identification by multiplex amplifying mtDNA-HV I, HV II and cytb regions is simple and sensitive. It is of use for forensic identification.

Animals↗