Biomedical subjects

Zhi-ying Yang

Publications and source records attributed to Zhi-ying Yang.

7 recordsLinked to original sources

PubMed2006

[One-day bowel preparation with sodium phosphate prior to colorectal surgery: a prospective, randomized, controlled clinical trial].

OBJECTIVES: To investigate the feasibility and safety of one-day bowel preparation for colorectal surgery. METHODS: Forty patients undergone colorectal surgery were divided randomly into the Control group and the Experimental group and received 3-day magnesium sulfate and 1-day sodium phosphate bowel preparations before the operation, respectively. The levels of hemoglobin, hematocrit, serum electrolytes, and anaerobe counts in the stool prior and post bowel preparation were examined. The general status, surgical complications, and structure of intestinal mucosa in the patients were observed after the operation. RESULTS: There was no significant difference in the anastomoses healing, infectious complications, serum tests and intestinal mucosa structures between the two groups. Less diarrhea occurred prior and post the surgery in the experimental group, and they felt better with the bowel preparation. The anaerobe counts in stool were higher after the bowel preparation than before in both groups. CONCLUSIONS: One-day bowel preparation with sodium phosphate is a safe and reliable method for colorectal surgery. The shortening of preparation time can reduce the degrees of uncomfortable feeling and disruptions of intestinal micro-ecology and barrier.

Colorectal Neoplasms↗

PubMed2006

[GPI-PLD gene exon14 polymorphisms of leucocyte in peripheral blood from healthy persons and leukemia patients].

OBJECTIVE: To analyze the polymorphisms of glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) gene exon 14, GPI-PLD activity of leucocyte in the peripheral blood,and the relationship in leukemia patients of Han nationality in Hunan. METHODS: Both 96 leukemia patients and 96 healthy persons of Han nationality in Hunan were researched [including 48 acute non-lymphocytic leukaemia (ANLL) patients as group A, 31 acute lymphoblastic leukaemia (ALL) patients as group B, 12 chronic granulocytic leukaemia (CML) patients as group C, 5 chronic lymphocytic leukaemia (CLL) patients as group D]. The polymorphisms were analyzed by PCR-SSCP and sequencing;. and GPI-PLD activities were determined by GPI-anchored placental alkaline phosphatase (PLAP) as substrate and triton-X114 partioning. RESULTS: There were four variations in the coverage of GPI-PLD gene exon 14 of leukemia patients and healthy persons. The codons of variation were: 1257 C-->T, 1298 T-->C, 1218 C-->A, 1257 C-->A. The total various frequency in leukemia patient and healthy person, which was determined by SSCP, was 28.12% and 20.83%. On the basis of the percentage of GPI-anchored PLAP conversion, the leucocyte GPI-PLD activities of the 96 leukemia patients were measured. Compared with the 96 healthy controls, the leukocyte GPI-PLD activites of ANLL and CLL patients were significantly increased; the acticities of ALL and CML patients were significantly reduced. CONCLUSION: Leukocyte GPI-PLD gene in the peripheral blood, which belongs to healthy persons and leukemia patients of Han nationality in Hunan, is polymorphism. The leukocyte GPI-PLD activities in the four groups are remarkable.

Adolescent↗

PubMed2006

[Diagnosis and treatment of primitive neuroectodermal tumors of pancreas].

OBJECTIVE: To improve the diagnosis and treatment of primitive neuroectodermal tumors (PNET) of the pancreas. METHODS: One patient with PNET of the pancreas was reported in this article. The corresponding literatures on the diagnosis and treatment was reviewed. RESULTS: The patient was diagnosed as pancreatic PNET by her clinical, microscopic, and immunohistochemical features as well as cytogenetic analysis after the resection of the tumor located in the uncinate process in PUMC Hospital. Radiochemotherapy was given after the operation for 8 months and no recurrence was observed. Since PNET of pancreas have no specific clinical symptoms and most patients have jaundice and/or abdominal pain, the diagnosis depended on the immunohistochemical features of positive P30/32(MIC2) and at least two of the neural markers. The cytogenetic analysis showed translocation mainly harbored the characteristic t (11; 22) (q24; q12). Since pancreatic PNET were highly aggressive, early chemotherapy, close follow-up, and immediate surgical interventions were required as early as possible. CONCLUSION: PNET can occur in pancreas, and diagnosis and treatment should be made as early as possible to improve the outcome.

Child↗

PubMed2005

[Influence on the cytokines expression on hepatic tissue by inhibition the signal pathway of inflammatory mediators following extensive hepatectomy in rats].

OBJECTIVE: To investigate the impact of AG490, a cytokine signaling inhibitor, on cytokine signaling pathway with phosphorylation levels of Janus kinase 2 (Jak2) and singal transducers and activators of transcription 3 (Stat3), and liver pro-/anti-inflammatory cytokine expressions. METHODS: Rats were divided into two groups after surgery: control group, without treatment; AG490 group, with AG490 (1 mg.kg(-1).12 h(-1)) administration intraperitoneally, immediately and through 36 hs after the operation. Western blotting was used to detect the levels of phosphorylated Jak2 and Stat3. Semi-quantitative RT-PCR was employed to examine Interleukin-6 (IL-6) and Interleukin-10 (IL-10) expression. RESULTS: At 8 h and 12 h post-operatively, the phosphorylation levels of Jak2 and Stat3 were significantly inhibited in the AG490 group when compared with the control group. The DNA levels of IL-6 in the liver of the AG490 group rat at the same time points were also decreased, whereas IL-10 levels markedly increased. These changes made the ratio of IL-6/IL-10 dropped significantly. CONCLUSIONS: AG490 ameliorates the overwhelming inflammatory response via a mechanism of blocking cytokine signaling transduction and consequently suppresses the ratio of pro-/anti-inflammatory cytokine expression, which exerts potential clinical implications of use of anti-inflammatory agents in hepatic surgery.

Animals↗

PubMed2005

[Mechanisms of increased survival rate by administration of AG490 in rats following extensive liver resection].

OBJECTIVE: To study the mechanism by which AG490 improves the survival rate of rats following extensive liver resection. METHODS: Thirty-eight rats were randomly divided into two groups after surgery: control group (n=10), without treatment; (2) AG490 group (n=28), with AG490 (1 mg x kg(-1) x 12 h(-1)) administrated intraperitoneally immediately and 36 hours after the operation. The survival rate was observed and the serum liver functions were measured. RESULTS: The survival rates of control group and AG490 group were 0% and 25%. AG490 group had significantly better blood glucose and aminotransferase levels (P < 0.05) than control group; serum bilirubin levels significantly decreased 48 hours after the operation. Serum protein levels in both two groups had slow decrease but without statistical significance (P > 0.05). CONCLUSIONS: AG490 can significantly increase the survival rate of rats following extensive liver resection. Such a benefit mainly results from the protection towards residual liver function rather than from the promotion of liver regeneration.

Alanine Transaminase↗

PubMed2004

[Effect of the expression of glycosylphosphatidylinositol-specific phospholipase D gene on K562 cell sensitivity to complement killing].

OBJECTIVE: To detect the release of glycosylphosphatidylinositol (GPI) anchored CD55 and CD59, and the effect of complement dependent cytotoxicity on K562 cell with expression of GPI-PLD by glucose or insulin. METHODS: CD55 and CD59 were detected by Western blotting; GPI-PLD activities were analyzed quantitatively; and complement dependent cytotoxicity (CDC) effects were observed by staining of trypan blue. RESULTS: After being induced by insulin or glucose for 24 h and 48 h, GPI-PLD activities and rate of CDC killing in the insulin, insulin + glucose groups significantly increased. At 24 h after the inducement, CD55 was found in the membrane proteins in the control, glucose and insulin groups and CD59 in membrane proteins in the control, glucose group, and medium supernatants of insulin, glucose + insulin groups. Both CD55 and CD59 were found in membrane proteins in control group, and medium supernatants of glucose, insulin, glucose + insulin group at 48 h after the inducement. CONCLUSION: Treatment with insulin resulted in the obvious increase of GPI-PLD activity in K562 cell, which led to releasing of GPI anchored CD55 and CD59 into medium, and increased sensitivity of these cells to CDC killing.

CD55 Antigens↗

PubMed2003

[Thyroid nodules with calcification and thyroid carcinoma].

OBJECTIVE: To investigate the significance of thyroid calcification for diagnosis of thyroid carcinoma. METHODS: Retrospective analysis of 817 thyroid nodules' pre-operative ultrasonic and postoperative pathologic results. RESULTS: Total ultrasonic thyroid calcification ratio was 18.1% (148/817). Total pathologic thyroid calcification ratio was 19.6% (160/817), which in benign samples was lower than that in malignant samples (13.1% vs 53.5%, P < 0.01). Micro-calcification ratio in benign samples was lower than that in malignant samples (2.9% vs 38.6%, P < 0.01). CONCLUSIONS: Thyroid nodules with calcification especially micro-calcification is considered to be the most specific sign of thyroid carcinoma, so the detection of it should be an important diagnostic criterion.

Adolescent↗