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Zhongsheng Wang

Publications and source records attributed to Zhongsheng Wang.

5 recordsLinked to original sources
PubMed2025

Evolutionary legacy of the "living fossil" genus Parrotia (Hamamelidaceae): genomic insights into species divergence and polygenic adaptation.

Despite their long evolutionary history, the genomic basis of adaptation and speciation in "living fossil" plants remain largely unexplored. Parrotia, a Tertiary relict tree genus with two extant species, P. subaequalis and P. persica, exhibits a disjunct distribution between East Asia and West Asia. Here, we present the first chromosome-level assemblies for both species, confirmed their sibling relationship, and dated the speciation event to the early Miocene. The recent proliferation of long-terminal repeat retrotransposons has driven the genome expansion in P. subaequalis. We detected widespread heterogeneous genomic differentiation between species. Extensive signals of divergent selection, local adaptation, and elevated Ka/Ks ratios in Parrotia indicate that this genus has undergone adaptive evolution in distinct refugia, challenging the notion of it as an "evolutionary dead end". Our findings provide new insights into the genomic evolution, environmental adaptation, and speciation of this "living fossil" tree genus.

Genome, Plant↗
PubMed2006

A dataset of human fetal liver proteome identified by subcellular fractionation and multiple protein separation and identification technology.

A high throughput process including subcellular fractionation and multiple protein separation and identification technology allowed us to establish the protein expression profile of human fetal liver, which was composed of at least 2,495 distinct proteins and 568 non-isoform groups identified from 64,960 peptides and 24,454 distinct peptides. In addition to the basic protein identification mentioned above, the MS data were used for complementary identification and novel protein mining. By doing the analysis with integrated protein, expressed sequence tag, and genome datasets, 223 proteins and 15 peptides were complementarily identified with high quality MS/MS data.

Cell Membrane↗
PubMed2006

Protein probabilities in shotgun proteomics: evaluating different estimation methods using a semi-random sampling model.

The calculation of protein probabilities is one of the most intractable problems in large-scale proteomic research. Current available estimating methods, for example, ProteinProphet, PROT_PROBE, Poisson model and two-peptide hits, employ different models trying to resolve this problem. Until now, no efficient method is used for comparative evaluation of the above methods in large-scale datasets. In order to evaluate these various methods, we developed a semi-random sampling model to simulate large-scale proteomic data. In this model, the identified peptides were sampled from the designed proteins and their cross-correlation scores were simulated according to the results from reverse database searching. The simulated result of 18 control proteins was consistent with the experimental one, demonstrating the efficiency of our model. According to the simulated results of human liver sample, ProteinProphet returned slightly higher probabilities and lower specificity than real cases. PROT_PROBE was a more efficient method with higher specificity. Predicted results from a Poisson model roughly coincide with real datasets, and the method of two-peptide hits seems solid but imprecise. However, the probabilities of identified proteins are strongly correlated with several experimental factors including spectra number, database size and protein abundance distribution.

Chromatography, Liquid↗
PubMed2003

[Influences of petrophytia moss on vegetation development in evergreen broad-leaved forest].

In order to examine the role of Petrophytia moss in maintaining the stability and integrity of forest vegetation, the distribution patterns of vascular plants among Petrophytia moss layer were investigated in five heterogeneous patches of evergreen broad-leaved forest at Longwangshan, Zhejiang Province. The distribution and composition of vascular plants were jointly affected by various factors, such as disturbance degree in patch, moss growth condition, and water and soil conservation ability of moss layer. Original habitats patch 1 and patch 5 were kept well, and hence, the even depth, dry weight and maximum water-holding capacity of moss layer, as well as the dry weight of soil and the soil water-absorbing rate in moss layer for patch 1 and patch 5 were much more than other patches. For example, the even depth (cm) of moss layer were decreased in the order of patch 5(2.2) > patch 1(2.0) > patch 2(1.5) > patch 3(1.1) > patch 4(0.9); the ranking of vascular plant diversities among moss layer in each patch was patch 5(16) > patch 1(14) > patch 3(9) > patch 4(7), and the general cover of these plants was followed as patch 3(30.0%) > patch 1(28.5%) > patch 5 (26.5%) > patch 2 (17.0%) > patch 4(4.5%). It was concluded that Petrophytia moss had the roles of reserving water and soil, holding litter, concentrating nutrient elements, and corrupting rock, which could improve the environmental condition of rock surface, help to the regeneration of vascular plants, and bring positive effects on the restoration or conversation of vegetation in disturbance sites and on the extension of forest scale.

Bryophyta↗