Biomedical subjects

Zuguo Liu

Publications and source records attributed to Zuguo Liu.

22 records · Page 2Linked to original sources

PubMed2002

An experimental study of anti-angiogenesis with recombinant human kringle 5.

OBJECTIVE: To investigate the anti-angiogenesis effects of recombinant human kringle 5 (rhk5) eye drops on corneal neovascularization (CNV) in rabbits induced by alkali burn. METHODS: Forty New Zealand albino rabbits were burned on the central corneas of their right eyes by 1 mol/L NaOH for 60s. Animals were randomly divided into 4 groups, 10 rabbits each group. Then rhk5 eye drops with different concentrations were applied four times daily for four weeks on each group: 5 mg/L in group 1, 10 mg/L in group 2, 20 mg/L in group 3 and carrier solution in group 4 (controls). The occurrence and development of CNV was observed every other day by slit-lamp microscope, and the area of CNV was calculated. Then the rabbits were killed on the 28th day (d), and the corneas were taken for histopathological examinations. Pearson's analysis was used to analyze the correlation between the number of inflammatory cells and the area of CNV. RESULTS: The occurrence of CNV in group 1 was (3.4 +/- 0.5) d, group 2 (6.8 +/- 0.4) d, group 3 (6.7 +/- 0.7) d and group 4 (3.7 +/- 0.5) d. Significant difference (P < 0.05) in the occurrence time of CNV was found between group 2 and the control. Compared with the control group, the CNV occurrence was significantly delayed in the group treated by 10 mg/L rhk5. No significant difference was found between control group and group 1, and between group 2 and group 3. The CNV areas of group 2 and group 3 were also smaller than the control group (P < 0.05). The area of CNV had close relationship with the retrocorneal membrane and with the number of inflammatory cells (P < 0.05). CONCLUSION: Topical application of recombinant human rhk5 has prominent effectiveness on the inhibition of angiogenesis induced by alkali burn.

Angiogenesis Inhibitors↗

PubMed2002

[Corneal topography and thickness in keratoconus].

OBJECTIVE: To evaluate the corneal topography and pachymetry in keratoconus. METHODS: Thirty-five patients (47 eyes) with keratoconus were evaluated by Orbscan corneal topography system. The location, anterior and posterior surface elevation, curvature and pachymetry were analyzed with special reference to the central point of cornea, the apex, the highest point of posterior surface, the point with greatest curvature and the thinnest point. The correlation between the point of greatest curvature and the apex was also analyzed. RESULTS: The mean elevation of the apex was (0.102 +/- 0.044) mm, the mean distance between the apex and the central point of cornea was (0.686 +/- 0.376) mm. Most of the apexes, the points with the highest posterior surface, the points with the greatest curvature were located in the inferior temporal quadrant, followed by inferior nasal, superior temporal and superior nasal quadrant. The SimK of keratocunus was (60.9 +/- 10.4/53.4 +/- 8.7) D and the mean powers within 3 mm and 5 mm were (57.1 +/- 8.0) D and (50.7 +/- 5.4) D respectively. High correlation between apex elevation and the apex curvature was found (r = 0.697, P < 0.01), and there was also negative relationship between apex elevation and corneal thickness (r = -0.563, P < 0.01). CONCLUSIONS: The Orbscan system can provide accurate and subtle information about the shape and thickness of keratoconus, which can help us to learn this disorder deeply, especially in the early diagnosis of this disease.

Adolescent↗

PubMed2002

[Expression of vascular endothelial growth factor and its receptors (flt-1) in morbid human corneas and investigation of its clinic importance].

PURPOSE: To investigate the expression of vascular endothelial growth factor (VEGF) and its receptor (flt-1) in morbid corneas and the potential effects. METHODS: Eleven cases of normal peripheral corneas and limbal tissues and 32 cases of miscellaneous morbid corneas were collected during corneal transplantation. The specimens were sectioned in a cryostat and stained with streptavidin-biotin-peroxidase method for VEGF and flt-1. The expressive levels of VEGF were compared with grades of patients' clinical manifestations. RESULTS: Positive expression of VEGF was exclusively presented in all morbid corneal specimens and that of flt-1 was showed in most cases. Stronger staining was found in the epithelia and stoma of the morbid cornea. The expressive levels of VEGF were more important in the stroma and the endothelia of the morbid corneas than that in the normal peripheral corneas and were positively correlated with the degrees of corneal opacities and the area of corneal neovascularization in infiltrating inflammatory cells and proliferative tissues ( P < 0. 05). CONCLUSIONS: VEGF and flt-1 were expressed in miscellaneous morbid corneas and may play an important role in the repair of corneal injuries and the corneal neovascularization.

Cornea↗

PubMedDate not supplied

Novel mutation (V505D) of the TGFBI gene found in a Chinese family with lattice corneal dystrophy, type I.

PURPOSE: To report a novel V505D mutation of the human transforming growth factor beta-induced (TGFBI) gene found in a Chinese family with lattice corneal dystrophy, type I (LCDI). METHODS: Genomic DNA was extracted from peripheral leukocytes from eight affected and four unaffected members of a Chinese family with LCDI. Exons of the TGFBI gene were amplified by polymerase chain reaction and directly sequenced. Fifty normal Chinese individuals were also analysed as controls. Histopathological examination of a corneal button was performed after keratoplasty of the proband. RESULTS: A heterozygous single-base-pair transversion (GTC to GAC, valine to aspartic acid) at codon 505 in exon 11 of the TGFBI gene (V505D) was detected in all of the affected members. No mutation was found in the unaffected members or in the 50 normal controls. The mutation cosegregated with the disease phenotype throughout three generations. Although a slit-lamp examination showed features of LCDI in most cases, the age at onset of the symptoms was several years later than that in cases of LCDI with an R124C mutation. By histopathological examination, numerous amyloid deposits were observed in the stroma, including beneath Bowman's membrane. CONCLUSION: A novel V505D mutation in the TGFBI gene causes LCDI in this Chinese family. It is the fourth reported mutation of the TGFBI gene associated with LCDI.

Adult↗