@misc{indiciaedfc520be8e93,
  title = {Single-step PCR optimization using touchdown and stepdown PCR programming.},
  author = {Kenneth H Roux},
  year = {2002},
  doi = {10.1385/1-59259-177-9:031},
  url = {https://pubmed.ncbi.nlm.nih.gov/12494634/},
  note = {Source identifier: 12494634}
}