A cis-acting A-U sequence element induces kinetoplastid U-insertions.

L M Brown; B J Burbach; B A McKenzie; G J Connell

doi:10.1074/jbc.274.10.6295

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A cis-acting A-U sequence element induces kinetoplastid U-insertions.

L M Brown·B J Burbach·B A McKenzie·G J Connell

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Abstract

A 34-nucleotide A-U sequence located immediately upstream of the editing sites of the Leishmania tarentolae cytochrome b mRNA induces a mitochondrial extract to insert U nucleotides independent of guide RNA. Insertions are localized to positions immediately 5' and 3' of the A-U sequence. When placed within an unedited mammalian transcript, the A-U sequence is sufficient to induce U-insertions. The sequence has a high degree of similarity with the templating nucleotides of a cytochrome b guide RNA and with a sequence adjacent to the editing sites in ND7 mRNA, the other characterized kinetoplastid mRNA supporting guide RNA-independent U-insertions. At least one protein specifically interacts with the A-U sequence. The reaction is consistent with a mechanism proposed for guide RNA-directed editing.

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Animals Base Sequence Cytochrome b Group DNA Transposable Elements DNA, Kinetoplast Leishmania Molecular Sequence Data RNA Editing RNA, Guide, Kinetoplastida RNA, Protozoan Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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L M Brown, B J Burbach, B A McKenzie, G J Connell. 1999-03-05. A cis-acting A-U sequence element induces kinetoplastid U-insertions.. https://doi.org/10.1074/jbc.274.10.6295

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