High resolution polyacrylamide gradient gel electrophoresis.

J J Esposito; J F Obijeski

doi:10.1080/00327487608069128

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PubMed · 1005359

High resolution polyacrylamide gradient gel electrophoresis.

J J Esposito·J F Obijeski

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Abstract

A method was developed for high resolution electrophoresis of proteins in linear gradient (3 to 30%) polyacrylamide gel rods in a neutral phosphate buffer containing 0.1% sodium dodecyl sulfate. Well-defined protein zones were observed and improved resolution was attained especially for low molecular weight proteins in preparations containing a variety of polypeptides, e.g. viruses that are often separated by continuous gel methods. Electropherograms of continuous (8%) and gradient (3 to 30%) gels were made of purified vesicular stomatitis virus, variola virus, Rickettsia rickettsii, and alpha and beta chains of hemoglobin in order to demonstrate the resolution of the gradient system.

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Acrylamides Electrophoresis, Polyacrylamide Gel Molecular Weight Peptides Proteins Species Specificity Viral Proteins Journal Article

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J J Esposito, J F Obijeski. 1976. High resolution polyacrylamide gradient gel electrophoresis.. https://doi.org/10.1080/00327487608069128

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