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PubMed · 10063537

Relationship between resistance to complement, virulence and outer membrane protein patterns in pathogenic Escherichia coli O2 isolates.

M Chaffer·E D Heller·B Schwartsburd
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Abstract

To establish a possible relationship between resistance to complement, virulence and outer membrane protein banding patterns, ten E. coli O2 strains isolated from chickens with colibacillosis were studied for: (1) resistance to the bactericidal effect of complement by a quantitative microtiter method, (2) virulence, as determined by chicken lethality test, and (3) outer membrane protein banding patterns yielded by SDS-polyacrylamide gel electrophoresis. The ten isolates were classified into three groups: (1) Group 1, consisting of four isolates showed: (a) high resistance to complement, (b) high virulence, and (c) different pattern between 35 and 40 kDa with a weak peptide band at 35 kDa. (2) Group 2, consisting of one isolate showed: (a) high resistance to complement, (b) low virulence, and (c) a weak peptide band at 35 kDa. (3) Group 3, consisting of five isolates showed: (a) low resistance to complement, (b) low virulence, and (c) identical OMP pattern between 35 and 40 kDa exhibiting a strong peptide band at 35 kDa. The results suggest that high resistance to complement may be necessary but no sufficient for virulence and that OMP banding patterns may be a marker for virulence.

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AnimalsBacterial Outer Membrane ProteinsBiomarkersChickensComplement Fixation TestsComplement System ProteinsElectrophoresis, Polyacrylamide GelEscherichia coliEscherichia coli InfectionsMalePoultry DiseasesVirulenceJournal ArticleResearch Support, Non-U.S. Gov't

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M Chaffer, E D Heller, B Schwartsburd. 1999-02-12. Relationship between resistance to complement, virulence and outer membrane protein patterns in pathogenic Escherichia coli O2 isolates.. https://doi.org/10.1016/s0378-1135(98)00278-8

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