Tryptic digest mapping by gradient capillary electrochromatography.

B Behnke; J W Metzger

doi:10.1002/(sici)1522-2683(19990101)20:1<80::aid-elps80>3.0.co;2-i

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PubMed · 10065962

Tryptic digest mapping by gradient capillary electrochromatography.

B Behnke·J W Metzger

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Abstract

A high performance liquid chromatography (HPLC) system complemented with T-split, capillary detection cell and a high voltage power supply was used for peptide mapping by gradient electrochromatography and nanoliquid chromatography (nano-LC). With capillary columns of 100 microm ID, 6 cm packed with octadecylated 1.5 microm silica particles, the typical analysis time was approximately 10-15 min. The resolution of a tryptic digest of cytochrome c obtained by electrochromatography at 100 kV/m was superior compared to the analysis by nano-LC. Bubble formation caused by Joule heating at currents up to 100 microA was successfully suppressed by using a resistor capillary of 25 microm ID connected to the outlet of the packed column.

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B Behnke, J W Metzger. 1999. Tryptic digest mapping by gradient capillary electrochromatography.. https://doi.org/10.1002/(sici)1522-2683(19990101)20%3A1%3C80%3A%3Aaid-elps80%3E3.0.co%3B2-i

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