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PubMed · 10068138

Electromigration for separations of protein complexes.

A Tulp·D Verwoerd·J Neefjes
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Abstract

This paper describes electromigration of complexes, consisting of two or more proteins and non-covalently associated peptides. Relatively small complexes (Mr < 1000000) can be resolved in sieving matrices. Large complexes are separated in free liquid systems. Examples of separation are given using native gels, denaturing gels and special formats thereof: blue native PAGE and gels incorporating a transversal temperature gradient. Both preparative and analytical applications are discussed as well as separations leading to mechanistic models of protein interaction. Carrier-free electrophoresis is represented by capillary zone electrophoresis, free-flow electrophoresis and density gradient electrophoresis. Emphasis is put on the free liquid separation of clathrin-coated vesicles and proteasomes.

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A Tulp, D Verwoerd, J Neefjes. 1999-02-05. Electromigration for separations of protein complexes.. https://doi.org/10.1016/s0378-4347(98)00504-0

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