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PubMed · 10086279

[Anticancer susceptibility test method using general-purpose dissolved oxygen meter].

J Arai·K Yamada·M Yasuda·M Yoshida·S Unten·M Akamatsu·H Ishikawa
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Abstract

Drug susceptibility of cell can be rapidly measured by continuously monitoring its metabolic changes. We focused on respiration volume as a signal of metabolic change, and developed a new dissolved oxygen measuring system which can detect respiration volume of cells. The main feature of the system is the use of a new type bare oxygen electrode which can easily detect the changing rate of dissolved oxygen concentration. In this study, single type electrode was used to evaluate this rapid anticancer drug susceptibility test. The result obtained was almost equivalent to that with MTT method, which is a conventional method for susceptibility test of HL-60 to various kinds of anticancer drugs. We have also developed multi-type electrode plate with oxygen electrodes embedded in the bottom of 96-well plate, with which clinical evaluation of this method can be easily made.

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Antineoplastic AgentsDrug Resistance, NeoplasmDrug Screening Assays, AntitumorElectrodesHL-60 CellsHumansEnglish AbstractJournal Article

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J Arai, K Yamada, M Yasuda, M Yoshida, S Unten, M Akamatsu, H Ishikawa. 1998. [Anticancer susceptibility test method using general-purpose dissolved oxygen meter].. https://pubmed.ncbi.nlm.nih.gov/10086279/

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