PubMed · 10088412
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K Hashiguchi, K Tomono, S Kohno. 1999. [Streptococcal pneumonia].. https://pubmed.ncbi.nlm.nih.gov/10088412/
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The rapid quantification of the enantiomers of small chiral molecules is very important, notably in pharmacology. Here, we show that the enantiomers of drug molecules can be distinguished by stochastic sensing, a single-molecule detection technique. The sensing element is an engineered alpha-hemolysin protein pore, fitted with a beta-cyclodextrin adapter. By using the approach, the enantiomeric composition of samples of ibuprofen and thalidomide can be determined in less than 1 s.
Stachybotrys chartarum is a toxigenic fungus that has been associated with human health concerns, including pulmonary hemorrhage and hemosiderosis. This fungus produces a hemolysin, stachylysin, which in its apparent monomeric form has a molecular mass of 11,920 Da as determined by matrix-assisted laser desorption ionization-time of flight mass spectrometry. However, it appears to form polydispersed aggregates, which confounds understanding of the actual hemolytically active form. Exhaustive dialysis or heat treatment at 60 degrees C for 30 min inactivated stachylysin. Stachylysin is composed of about 40% nonpolar amino acids and contains two cysteine residues. Purified stachylysin required more than 6 h to begin lysing sheep erythrocytes, but by 48 h, lysis was complete. Stachylysin also formed pores in sheep erythrocyte membranes.
Sixty marine water samples were collected from various coastal sites around Port Blair at different times during August 1996 to July 1997. The specimens were subjected to standard procedure for isolation and identification of V. parahaemolyticus. Forty four V. parahaemolyticus isolates were detected from these specimens and all showed clear haemolysis on Wagatsuma agar plates. The haemolytic activity was abolished by heating the culture supernatants at 60 degrees C for 10 min and enhanced when plates were kept at 4 degrees C. When isolates were subjected to PCR assay for tdh gene, only one showed the presence of the gene. The results indicate the existence of pathogenic V. parahaemolyticus in the marine environment of these islands.