PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 10092513

Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity.

Y Arita·S Kihara·N Ouchi·M Takahashi·K Maeda·J Miyagawa·K Hotta·I Shimomura·T Nakamura·K Miyaoka·H Kuriyama·M Nishida·S Yamashita·K Okubo·K Matsubara·M Muraguchi·Y Ohmoto·T Funahashi·Y Matsuzawa
View original source ↗Export metadata ↓

Abstract

We isolated the human adipose-specific and most abundant gene transcript, apM1 (Maeda, K., et al., Biochem. Biophys. Res. Commun. 221, 286-289, 1996). The apM1 gene product was a kind of soluble matrix protein, which we named adiponectin. To quantitate the plasma adiponectin concentration, we have produced monoclonal and polyclonal antibodies for human adiponectin and developed an enzyme-linked immunosorbent assay (ELISA) system. Adiponectin was abundantly present in the plasma of healthy volunteers in the range from 1.9 to 17.0 mg/ml. Plasma concentrations of adiponectin in obese subjects were significantly lower than those in non-obese subjects, although adiponectin is secreted only from adipose tissue. The ELISA system developed in this study will be useful for elucidating the physiological and pathophysiological role of adiponectin in humans.

Explore related subjects

AdiponectinAdipose TissueAdultAnimalsAntibodiesBlotting, WesternBody Mass IndexEnzyme-Linked Immunosorbent AssayEscherichia coliFemaleHot TemperatureHumansIntercellular Signaling Peptides and ProteinsLinear ModelsMaleMiceMiddle AgedMolecular WeightObesityProtein BindingProtein Sorting SignalsProteinsRabbitsRecombinant ProteinsJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Y Arita, S Kihara, N Ouchi, M Takahashi, K Maeda, J Miyagawa, K Hotta, I Shimomura, T Nakamura, K Miyaoka, H Kuriyama, M Nishida, S Yamashita, K Okubo, K Matsubara, M Muraguchi, Y Ohmoto, T Funahashi, Y Matsuzawa. 1999-04-02. Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity.. https://doi.org/10.1006/bbrc.1999.0255

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed1999

Organization of the gene for gelatin-binding protein (GBP28).

GBP28 is a novel human plasma gelatin-binding protein that is encoded by apM1 mRNA, expressed specifically in adipose tissue. Three overlapping clones (two lambda clones and one BAC clone) containing the human plasma gelatin-binding protein (GBP28) gene were isolated and characterized. The GBP28 gene spans 16kb and is composed of three exons from 18bp to 4277bp in size with consensus splice sites. The sizes of the two introns were 0.8 and 12kb, respectively. The gene's regulatory sequences contain putative promoter elements, but no typical TATA box. The third exon of this gene contains a long 3'-untranslated sequence containing three Alu repeats. The exon-intron organization of this gene was very similar to that of obese gene, encoding leptin. We also report the chromosome mapping of this gene by fluorescence in situ hybridization (FISH) using a genomic DNA fragment as a probe. The GBP28 gene was located on human chromosome 3q27. The nucleotide sequence data reported in this paper will appear in the DDBJ/EMBL/GenBank nucleotide sequence databases with the accession numbers ABO12163, ABO12164 or ABO12165.

Adiponectin↗
PubMed1999

Role of adipocytokines on the pathogenesis of atherosclerosis in visceral obesity.

Obesity which is defined as accumulation of excess body fat, is a major cause of atherosclerotic vascular disease in industrial countries. Recent advances in the biology of adipose tissue have revealed that adipose tissue is not simply an energy storage organ but it also secretes a variety of molecules which affect the metabolism of the whole body. Through a systematic search of active genes in adipose tissue, we found that adipose tissue, especially visceral fat expressed numerous genes for secretory proteins (about 30% of total genes analyzed). Among them, plasminogen activator-1 (PAI-1), which is a regulator of the fibrinolytic system, was overexpressed in the visceral fat in an animal model of obesity. Plasma levels of PAI-1 were closely correlated with visceral fat adiposity. Thus, PAI-1 secreted from visceral fat may play some role in thrombotic vascular disease in visceral obesity. Adiponectin, a novel adipose-specific gene product, which has a matrix-like structure, is abundantly present in the bloodstream. Dysregulated secretion of adiponectin may be related to vascular disease in obesity. Biologically active molecules secreted from adipose tissue (adipocytokines) may have important roles in the development of atherosclerotic disease in obesity.

Adiponectin↗