Improved sensitivity of reverse transcriptase polymerase chain reaction for hepatitis C virus using random hexamer primers.

S Radhakrishnan; P Abraham; G Sridharan

doi:10.1016/s0732-8893(98)00152-7

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Improved sensitivity of reverse transcriptase polymerase chain reaction for hepatitis C virus using random hexamer primers.

S Radhakrishnan·P Abraham·G Sridharan

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Abstract

The use of specific primers and random primers for the detection of hepatitis C virus RNA by reverse transcriptase-polymerase chain reaction was compared. The cDNA obtained from using both methods was amplified by the same protocol. Of the 30 plasma specimens tested, 6 (20%) were hepatitis C virus RNA-positive using specific primers and 19 (63%) were positive using random primers, demonstrating that the use of random hexamer-primed reverse transcriptase-polymerase chain reaction significantly improves the sensitivity (p < 0.01).

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DNA Primers DNA, Complementary Hepacivirus Hepatitis C Humans RNA, Viral Reverse Transcriptase Polymerase Chain Reaction Sensitivity and Specificity Journal Article

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S Radhakrishnan, P Abraham, G Sridharan. 1999. Improved sensitivity of reverse transcriptase polymerase chain reaction for hepatitis C virus using random hexamer primers.. https://doi.org/10.1016/s0732-8893(98)00152-7

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