Enzyme array-amperometric detection in carbohydrate analysis.

M Sun; C S Lee

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PubMed · 10099233

Enzyme array-amperometric detection in carbohydrate analysis.

M Sun·C S Lee

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Abstract

The introduction of an enzyme array-electrochemical detection method for carbohydrate analysis is demonstrated by using two complex and one high mannose N-linked oligosaccharides. Instead of measuring the remaining uncleaved oligosaccharides in enzymatic digestion, released monosaccharides are directly quantified by pulsed amperometric detection at a gold electrode. The measured monosaccharide concentrations in combination with the enzyme array analysis provide structural characterization of oligosaccharides. The enzyme array-electrochemical detection method does not require any separation procedure or any prior labeling of oligosaccharides. However, this method is limited by the use of purified oligosaccharide samples and the nature of the enzyme array. The development of more sophisticated enzyme arrays relies upon the introduction of a bank of highly specific (bond, arm, aglycon) exoglycosidases.

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Carbohydrate Metabolism Carbohydrate Sequence Carbohydrates Chromatography, Ion Exchange Electrochemistry Glycoside Hydrolases Molecular Sequence Data Oligosaccharides Sensitivity and Specificity Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

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M Sun, C S Lee. 1998-03-05. Enzyme array-amperometric detection in carbohydrate analysis.. https://pubmed.ncbi.nlm.nih.gov/10099233/

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