PubMed · 10146359
The source did not provide an abstract. Follow the original record for more information.
Explore connections, maps & timelines
M Kaback. 1993. Screening tests: problems with DNA-based insurance testing.. https://pubmed.ncbi.nlm.nih.gov/10146359/
Cite the original work for its findings. Save a collection to share your selection of sources.
KEEP EXPLORING
STALARD (selective target amplification for low-abundance RNA detection) enables isoform-level quantification of low-abundance RNAs using conventional laboratory equipment. Here, we describe steps for RNA isolation, primer design, reverse transcription, selective target amplification, and downstream analysis. The protocol couples selective pre-amplification with a quantitative reverse-transcription PCR (RT-qPCR) readout and optional nanopore sequencing. Using 1 μg input RNA and 12 pre-amplification cycles, STALARD reduces Cq values by approximately 10-12 cycles, bringing the target into a reliably quantifiable range. For complete details on the use and execution of this protocol, please refer to Jeong et al.1.
Metabolic labeling of HepG2 cells with 75selenite indicated the expression of more than four selenoproteins in the 80 to 45 kDa range and more than four selenoproteins in the 24 to 14 kDa range. Although there were no significant changes in the total protein content, determined by the method of BioRad, the densitometric analysis of the autoradiogramms showed a reduced expression of all labeled selenoproteins in the cell lysates of HepG2 after cytokine treatment for 24 hours. A stronger reduction was observed after treatment with Il-1beta than with IFNgamma. Moreover, the inhibitory effects were more significant for those selenoproteins in the higher molecular mass range. Our data on the inhibition of selenoprotein synthesis and on repression of SeP promoter activity show that the expression of selenoproteins, especially of SeP, is influenced by acute phase reaction and pro-inflammatory reactions.
Explore the source record for details and available documents.