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PubMed · 1016583

An improved method for PHA-culturing mouse lymphocytes using Ficoll-Hypaque gradient.

L C Giraudo Conesa·L Rumi·C D Pasqualini
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Abstract

A simple, reproducible method is described for in vitro culture of mouse lymphocytes purified by differential centrifugation through Ficoll-Hypaque gradient. Peripheral blood, spleen and thymus purified lymphocytes responded well to in vitro PHA stimulation as measured by blast transformation and tritiated thymidine incorporation; the lowest values were observed with thymus lymphocytes.

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AnimalsAntibody FormationCells, CulturedCentrifugationLectinsLymphocyte ActivationLymphocytesMaleMiceSpleenThymus GlandJournal Article

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L C Giraudo Conesa, L Rumi, C D Pasqualini. 1976-09-30. An improved method for PHA-culturing mouse lymphocytes using Ficoll-Hypaque gradient.. https://pubmed.ncbi.nlm.nih.gov/1016583/

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