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PubMed · 10191987

Nucleus extraction from single mounted tissue sections.

T Liehr·C Uwe·G Erich
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Abstract

Extraction of nuclei from unmounted archived tissue has become a method widely used for molecular cytogenetic investigations. It is a suitable approach to take advantage of the large series of formalin fixed and subsequently paraffin-embedded material which has been collected in the times before interphase cytogenetic analysis has become possible. We present a new kind of assay for the extraction of nuclei from one single mounted tissue section; the extracted interphase nuclei are suitable very well for a fluorescence in situ hybridization (FISH) approach. The method described has been successfully used for the analysis of the INT2/FGF3-amplicon in 20 samples of oral squamous cell carcinoma.

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Carcinoma, Squamous CellCell ExtractsCell NucleusFibroblast Growth Factor 3Fibroblast Growth FactorsHumansIn Situ Hybridization, FluorescenceMicrotomyMouth NeoplasmsProto-Oncogene ProteinsJournal ArticleResearch Support, Non-U.S. Gov't

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T Liehr, C Uwe, G Erich. 1999. Nucleus extraction from single mounted tissue sections.. https://doi.org/10.1016/s1050-3862(98)00037-0

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