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PubMed · 10198033

Cyclic AMP can decrease expression of genes subject to catabolite repression in Saccharomyces cerevisiae.

O Zaragoza·C Lindley·J M Gancedo
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Abstract

External cyclic AMP (cAMP) hindered the derepression of gluconeogenic enzymes in a pde2 mutant of Saccharomyces cerevisiae, but it did not prevent invertase derepression. cAMP reduced nearly 20-fold the transcription driven by upstream activation sequence (UAS1FBP1) from FBP1, encoding fructose-1,6-bisphosphatase; it decreased 2-fold the activation of transcription by UAS2FBP1. Nuclear extracts from cells derepressed in the presence of cAMP were impaired in the formation of specific UASFBP1-protein complexes in band shift experiments. cAMP does not appear to act through the repressing protein Mig1. Control of FBP1 transcription through cAMP is redundant with other regulatory mechanisms.

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Carboxy-LyasesCyclic AMPEnzyme RepressionExonucleasesFructose-BisphosphataseGene Expression Regulation, FungalGluconeogenesisGlutamate DehydrogenaseProtein BindingRegulatory Sequences, Nucleic AcidSaccharomyces cerevisiaeTranscription FactorsJournal ArticleResearch Support, Non-U.S. Gov't

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O Zaragoza, C Lindley, J M Gancedo. 1999. Cyclic AMP can decrease expression of genes subject to catabolite repression in Saccharomyces cerevisiae.. https://doi.org/10.1128/jb.181.8.2640-2642.1999

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