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PubMed · 10207886

Chiral discrimination using an immunosensor.

O Hofstetter·H Hofstetter·M Wilchek·V Schurig·B S Green
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Abstract

Based on the stereoselectivity of immunoglobulins, we have developed a new chiral sensor for the detection of low-molecular-weight analytes. Using surface plasmon resonance detection, enantiomers of free, underivatized alpha-amino acids can be monitored in a competitive assay by their interaction with antibodies specific for the chiral center of this class of substances. The sensitivity to the minor enantiomer in nonracemic mixtures exceeds currently available methods; therefore, such immunosensors can readily detect traces of enantiomeric impurities and are attractive for a range of applications in science and industry.

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Amino AcidsAnimalsAntibodiesAntigen-Antibody ReactionsImmunoglobulinsRabbitsSensitivity and SpecificityStereoisomerismSurface Plasmon ResonanceJournal ArticleResearch Support, Non-U.S. Gov't

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O Hofstetter, H Hofstetter, M Wilchek, V Schurig, B S Green. 1999. Chiral discrimination using an immunosensor.. https://doi.org/10.1038/7927

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