Modifying secretion and post-translational processing in insect cells.

E Ailor; M J Betenbaugh

doi:10.1016/s0958-1669(99)80024-x

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Modifying secretion and post-translational processing in insect cells.

E Ailor·M J Betenbaugh

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Abstract

The baculovirus-insect cell system is a valuable tool for the expression of heterologous proteins. Due to limitations in the intracellular processing environment, however, heterologous secreted and membrane proteins are often insoluble, poorly processed, or contain 'non-human' modifications. Recent attempts to modify the insect cell secretory pathway by overexpressing processing factors have demonstrated the potential to overcome these limitations.

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Animals Baculoviridae Cloning, Molecular Cytosol Endoplasmic Reticulum Insecta Molecular Chaperones Oligosaccharides Protein Processing, Post-Translational Protein Sorting Signals Journal Article Review

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E Ailor, M J Betenbaugh. 1999. Modifying secretion and post-translational processing in insect cells.. https://doi.org/10.1016/s0958-1669(99)80024-x

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