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A capillary-array electrophoresis system using side-entry on-column laser irradiation combined with glass rod lenses.

T Anazawa·S Takahashi·H Kambara
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Abstract

We have developed a simple and high-throughput capillary-array electrophoresis system that uses side-entry on-column laser irradiation. The number of capillaries in an array is generally limited by laser-power attenuation along the array due to reflection and divergence. We overcame these problems by placing the capillaries in water and adding glass rod lenses between the capillaries. As a result, up to 45 capillaries could be simultaneously irradiated with a single laser beam and the fluorescence from all the capillaries could be detected with high sensitivity. We demonstrated the high throughput of 12 kbp/h with a 45 capillary array using this system.

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Electrophoresis, CapillaryEvaluation Studies as TopicLasersJournal Article

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T Anazawa, S Takahashi, H Kambara. 1999. A capillary-array electrophoresis system using side-entry on-column laser irradiation combined with glass rod lenses.. https://doi.org/10.1002/(sici)1522-2683(19990301)20%3A3%3C539%3A%3Aaid-elps539%3E3.0.co%3B2-3

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