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PubMed · 10230856

Chronic hepatitis in interferon-gamma transgenic mice is associated with elevated CPP32-like activity and interleukin-1beta-converting enzyme activity suppression.

T Okamoto·Y Nakano·T Yamakawa·K Hara·K I Yamamura·O Hino
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Abstract

Interferon-gamma (IFN-gamma) transgenic mice strongly express IFN-gamma in the liver and develop chronic hepatitis. Furthermore, hepatocyte apoptosis was shown by the TdT-mediated dUTP-biotin nick endlabeling method. In the present study, interleukin-1beta-converting enzyme (ICE) and CPP32-like protease activities in the liver of IFN-gamma transgenic mice were measured, using the synthetic substrates Ac-YVAD-MCA and Ac-DEVD-MCA. Plasma aspartate aminotransferase and alanine aminotransferase activities as well as CPP32-like activity were significantly elevated, while ICE activity was significantly reduced. The addition of the ICE inhibitor Ac-YVAD-CHO to IFN-gamma transgenic mouse liver cell cytosol had no effect on the CPP32 activity, in contrast to a CPP32 inhibitor. The present results indicate that chronic hepatitis in the IFN-gamma transgenic mouse is associated with a decrease in ICE and induction of CPP32-like activity.

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Alanine TransaminaseAnimalsAspartate AminotransferasesCaspase 1Caspase 3CaspasesGene Expression RegulationHepatitis, AnimalHepatitis, ChronicIn Situ Nick-End LabelingInterferon-gammaLiverMiceMice, Inbred C57BLMice, TransgenicRNA, MessengerTumor Necrosis Factor-alphaJournal Article

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T Okamoto, Y Nakano, T Yamakawa, K Hara, K I Yamamura, O Hino. 1999. Chronic hepatitis in interferon-gamma transgenic mice is associated with elevated CPP32-like activity and interleukin-1beta-converting enzyme activity suppression.. https://doi.org/10.1254/jjp.79.289

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