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PubMed · 10231092

An scFv phage clone that binds to human beta2-microglobulin.

B Jwang·N Yerushalmi·R J Kreitman·J Engberg·I Pastan
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Abstract

Using phage display technology, a novel mouse scFv phage clone D1 was generated from mice immunized with single-chain HLA-A2, the human MHC class I molecules. D1 recognized a determinant in beta2-microglobulin. It reacted with beta2-microglobulin associated with the heavy chain of HLA-A2, recombinant beta2-microglobulin made in Escherichia coli or beta2-microglobulin prepared from human urine. The heavy chain variable region of D1 belongs to gene family VII and the light chain variable region belongs to the kappa chain gene family XI according to the Kabat database.

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Amino Acid SequenceAnimalsBacteriophagesCloning, MolecularEnzyme-Linked Immunosorbent AssayEscherichia coliHLA-A2 AntigenHumansImmunoglobulin FragmentsImmunoglobulin Heavy ChainsImmunoglobulin Light ChainsImmunoglobulin Variable RegionMiceMice, TransgenicMolecular Sequence DataPeptide LibraryRecombinant Proteinsbeta 2-MicroglobulinJournal Article

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B Jwang, N Yerushalmi, R J Kreitman, J Engberg, I Pastan. 1999. An scFv phage clone that binds to human beta2-microglobulin.. https://doi.org/10.1016/s1380-2933(98)00023-2

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