PubMed · 10298438
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1982. Chromatography.. https://pubmed.ncbi.nlm.nih.gov/10298438/
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The accuracy of the method of elution by characteristic point (ECP) used to determine single component isotherms was studied numerically. Overloaded elution peaks were calculated using the equilibrium-dispersive model of nonlinear chromatography while varying the four parameters, i.e., the number of theoretical plates, the dimensionless Langmuir equilibrium constant, the retention and the loading factor, involved in the fundamental equations of the problem. Single component isotherms were estimated by analyzing the diffuse profile of the elution peaks by the ECP method. Similar results were obtained with the transport-dispersive model. The comparison of these calculated isotherms with the initial Langmuir isotherms provided detailed information on the influence of the mass transfer resistances on the accuracy of the isotherms afforded by the ECP method. The systematic error made on the isotherms depends on the experimental conditions, described by the four parameters. It is expected that this error could be eliminated by correcting the influence of nonequilibrium in the column on the basis of results of the nondimensional calculations. The concentration range of the objective isotherm which can be determined by the ECP method can also be predicted by numerical calculations. The usefulness of the correction strategy and the prediction of the concentration range were experimentally demonstrated.
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The paper published by Ghaoui and Rothman [J. High Resolut. Chromatogr. 15 (1992) 36] and particularly its Fig. 5, is further considered here because it contains the germ of an idea of how to measure improvements to a chromatographic method, and how to define the goal of perfection in terms of "zero defects" as required by quality assurance schemes. From this, a new role emerges for signal averaging in capillary chromatography: a role to quantify and measure method improvements, and one which can be generally applied to measure improvements in instrument design too.