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PubMed · 10330125

An automated sample preparation system for large-scale DNA sequencing.

A Marziali·T D Willis·N A Federspiel·R W Davis
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Abstract

Recent advances in DNA sequencing technologies, both in the form of high lane-density gels and automated capillary systems, will lead to an increased requirement for sample preparation systems that operate at low cost and high throughput. As part of the development of a fully automated sequencing system, we have developed an automated subsystem capable of producing 10,000 sequence-ready ssDNA templates per day from libraries of M13 plaques at a cost of $0.29 per sample. This Front End has been in high throughput operation since June, 1997 and has produced > 400,000 high-quality DNA templates.

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DNASequence Analysis, DNAJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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A Marziali, T D Willis, N A Federspiel, R W Davis. 1999. An automated sample preparation system for large-scale DNA sequencing.. https://pubmed.ncbi.nlm.nih.gov/10330125/

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