[A new universal gene replacement method for generating a desired mutant mouse].

K Itoh

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PubMed · 10339930

[A new universal gene replacement method for generating a desired mutant mouse].

K Itoh

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Abstract

Site-directed gene replacement has been accomplished using two steps of gene targeting, which is called universal gene replacement. The previously described universal gene replacement method with the "neo-tk" cassette exhibited a very low efficiency in the second "replacement" step. The neo-tk tagged allele obtained from the first step was never transmitted to offspring through the germ cells of chimeric mice. We therefore developed a new system using the Eco-gpt gene instead of the HSV-tk gene. With this system, gene replacement for the p53 gene was thus consistently accomplished and generated mutant mouse lines with site-directed mutations. Further, all manipulated alleles, including the neo-gpt tagged one, were successfully transmitted to offspring.

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Animals Chimera Gene Targeting Genes, p53 Genetic Techniques Mice Recombination, Genetic English Abstract Journal Article

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K Itoh. 1999. [A new universal gene replacement method for generating a desired mutant mouse].. https://pubmed.ncbi.nlm.nih.gov/10339930/

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