Dual specificity antibodies using a double-stranded oligonucleotide bridge.

Z N Chaudri; M Bartlet-Jones; G Panayotou; T Klonisch; I M Roitt; T Lund; P J Delves

doi:10.1016/s0014-5793(99)00466-4

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PubMed · 10350050

Dual specificity antibodies using a double-stranded oligonucleotide bridge.

Z N Chaudri·M Bartlet-Jones·G Panayotou·T Klonisch·I M Roitt·T Lund·P J Delves

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Abstract

The covalent conjugation of oligonucleotides to antibody Fab' fragments was optimized by using oligonucleotides modified with a hexaethylene linker arm bearing three amino groups. One oligonucleotide was coupled to antibody of one specificity and a complementary oligonucleotide to antibody of a second specificity. The antibodies were then allowed to hybridize by base pairing of the complementary nucleotide sequences and the generation of bispecific antibody was analyzed on SDS-PAGE and confirmed using BIAcore analysis. The strategy of complementary oligonucleotide-linked bispecific molecules is not limited to antibodies but is applicable to linking any two molecules of different characteristics.

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Animals Antibodies, Bispecific Antibody Specificity Biosensing Techniques DNA Electrophoresis, Polyacrylamide Gel Humans Immunoglobulin Fab Fragments Mice Nucleic Acid Hybridization Oligonucleotides Journal Article Research Support, Non-U.S. Gov't

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Z N Chaudri, M Bartlet-Jones, G Panayotou, T Klonisch, I M Roitt, T Lund, P J Delves. 1999-04-30. Dual specificity antibodies using a double-stranded oligonucleotide bridge.. https://doi.org/10.1016/s0014-5793(99)00466-4

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