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Ornithine alpha-ketoglutarate counteracts the decrease of liver cytochrome P-450 content in burned rats.

M Roch-Arveiller·J Fontagné·C Coudray-Lucas·J Le Boucher·L Cynober·O Muntaner·M Delaforge
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Abstract

The effect of ornithine alpha-ketoglutarate (OKG) on cytochrome P-450 enzyme activities was studied in a well-defined model of injury (burn followed by fasting then subsequent hypocaloric diet) administered to young rats for 3 d. Hepatic microsomes were prepared by ultracentrifugation and levels of cytochromes P-450 were determined spectrophotometrically. The activities of ethoxy-resorufin-O-deethylase (EROD), benzyloxy-resorufin-O-dealkylase (BROD), and erythromycin demethylase were measured as markers of P-450 1A, 2A, and 3A isotypes respectively. The level of total hepatic microsomal proteins (8 mg/mL) remained constant. The level of cytochrome P-450 (1.14+/-0.08 nmol/mg microsomal proteins) was decreased by a hypocaloric diet (23%, P = 0.003) and burn further enhanced this phenomenon (15%, P = 0.03). Both healthy and burned rats receiving OKG showed the same level of cytochrome P-450 as the rats fed ad libitum. OKG supplementation counteracted the enhancement (40%) of EROD activity induced by hypocaloric diet but did not influence BROD and erythromycin demethylase activities. OKG sustained cytochrome P-450 levels in rats fed a hypocaloric diet, even after burning. These findings indicate that OKG may favor drug metabolism in this injured population.

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AnimalsAryl Hydrocarbon HydroxylasesBurnsCytochrome P-450 CYP1A1Cytochrome P-450 CYP2B1Cytochrome P-450 CYP3ACytochrome P-450 Enzyme SystemEnergy IntakeMaleMicrosomes, LiverOrnithineOxidoreductases, N-DemethylatingRatsRats, Sprague-DawleyJournal ArticleResearch Support, Non-U.S. Gov't

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M Roch-Arveiller, J Fontagné, C Coudray-Lucas, J Le Boucher, L Cynober, O Muntaner, M Delaforge. 1999. Ornithine alpha-ketoglutarate counteracts the decrease of liver cytochrome P-450 content in burned rats.. https://doi.org/10.1016/s0899-9007(99)00031-3

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