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PubMed · 10370237

Optical mapping and its potential for large-scale sequencing projects.

C Aston·B Mishra·D C Schwartz
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Abstract

Physical mapping has been rediscovered as an important component of large-scale sequencing projects. Restriction maps provide landmark sequences at defined intervals, and high-resolution restriction maps can be assembled from ensembles of single molecules by optical means. Such optical maps can be constructed from both large-insert clones and genomic DNA, and are used as a scaffold for accurately aligning sequence contigs generated by shotgun sequencing.

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Base SequenceBlotting, SouthernCloning, MolecularDNAGenome, HumanHumansMolecular Sequence DataOptics and PhotonicsSequence Analysis, DNAJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.Review

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C Aston, B Mishra, D C Schwartz. 1999. Optical mapping and its potential for large-scale sequencing projects.. https://doi.org/10.1016/s0167-7799(99)01326-8

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