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PubMed · 10374700

Human decidualized endometrial T lymphocytes do not substantially down-regulate CD3zeta.

I Bedenicki·D J Newton·B F Flanagan·P M Johnson·D Rukavina·S E Christmas
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Abstract

PROBLEM: The T-cell antigen receptor (TCR) has been reported to be down-regulated on T-cells in the decidualized endometrium in early pregnancy. METHOD OF STUDY: The expression of CD3zeta, a component of the TCR complex, has been investigated in human first-trimester decidual T-cells using flow cytometric analysis of permeabilized cells. RESULTS: Levels of CD3zeta expression were significantly lower in decidual than in peripheral T-cells from non-pregnant women, as assessed by mean fluorescence intensity (4.2 vs. 5.5, logarithmic scale, P < 0.05). However, when decidual and peripheral T-cells from the same subjects were analyzed (n = 10), mean levels of CD3zeta were slightly, but not significantly, lower in decidual than in peripheral T-cells (P > 0.1). CD3zeta was not substantially down-regulated systemically as mean cytoplasmic CD3zeta levels did not differ significantly between peripheral blood T-cells from pregnant women and non-pregnant controls (P > 0.2). CD8+ cells outnumber CD4+ cells in decidua, but neither the proportions of these two T-cell subsets positive for cytoplasmic CD3zeta nor the mean levels of CD3zeta were significantly different. CONCLUSIONS: These results indicate that human decidual T-cells do not greatly down-regulate CD3zeta, but it is unclear if a small decrease in mean levels may be sufficient to compromise their capacity for activation.

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CD3 ComplexDeciduaDown-RegulationFemaleFlow CytometryHumansLeukocytes, MononuclearPregnancyPregnancy Trimester, FirstReceptors, Antigen, T-CellT-LymphocytesJournal ArticleResearch Support, Non-U.S. Gov't

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I Bedenicki, D J Newton, B F Flanagan, P M Johnson, D Rukavina, S E Christmas. 1999. Human decidualized endometrial T lymphocytes do not substantially down-regulate CD3zeta.. https://doi.org/10.1111/j.1600-0897.1999.tb00434.x

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