DNA delivery from polymer matrices for tissue engineering.

L D Shea; E Smiley; J Bonadio; D J Mooney

doi:10.1038/9853

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PubMed · 10385318

DNA delivery from polymer matrices for tissue engineering.

L D Shea·E Smiley·J Bonadio·D J Mooney

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Abstract

We have proposed engineering tissues by the incorporation and sustained release of plasmids encoding tissue-inductive proteins from polymer matrices. Matrices of poly(lactide-co-glycolide) (PLG) were loaded with plasmid, which was subsequently released over a period ranging from days to a month in vitro. Sustained delivery of plasmid DNA from matrices led to the transfection of large numbers of cells. Furthermore, in vivo delivery of a plasmid encoding platelet-derived growth factor enhanced matrix deposition and blood vessel formation in the developing tissue. This contrasts with direct injection of the plasmid, which did not significantly affect tissue formation. This method of DNA delivery may find utility in tissue engineering and gene therapy applications.

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Animals Biocompatible Materials Cell Line DNA Gene Transfer Techniques Humans Lactic Acid Plasmids Platelet-Derived Growth Factor Polyglycolic Acid Polylactic Acid-Polyglycolic Acid Copolymer Polymers Rats Rats, Inbred Lew Journal Article Research Support, U.S. Gov't, P.H.S.

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L D Shea, E Smiley, J Bonadio, D J Mooney. 1999. DNA delivery from polymer matrices for tissue engineering.. https://doi.org/10.1038/9853

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