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Studies on conditional gene expression in the brain.

J Jerecic·F Single·U Krüth·H Krestel·R Kolhekar·T Storck·K Kask·M Higuchi·R Sprengel·P H Seeburg
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Abstract

This manuscript summarizes our recent attempts to regulate in vitro and in vivo the expression of genes encoding components and regulators of the postsynaptic machinery along with marker genes such as lacZ and GFP. In particular, we studied tTA-dependent regulation and utilized Cre in combination with reversible silencing by intron engineering of dominant negative alleles. We further present a "knockin" approach for on-site artificial regulation of chromosomal genes.

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AnimalsBrainCalcium-Calmodulin-Dependent Protein Kinase Type 2Calcium-Calmodulin-Dependent Protein KinasesGene Expression RegulationGenes, ReporterIntegrasesMiceMice, TransgenicRecombination, GeneticSynaptic TransmissionTetracyclineTrans-ActivatorsViral ProteinsJournal ArticleResearch Support, Non-U.S. Gov't

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J Jerecic, F Single, U Krüth, H Krestel, R Kolhekar, T Storck, K Kask, M Higuchi, R Sprengel, P H Seeburg. 1999-04-30. Studies on conditional gene expression in the brain.. https://doi.org/10.1111/j.1749-6632.1999.tb11271.x

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