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Islet cryopreservation protocols.

R V Rajotte
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Abstract

Low temperature banking of islets has facilitated ongoing clinical trials, allowing for the collection and long-term storage of islets during which viability and sterility assessment can be carried out. Islets from most species of animals have been cryopreserved using various freeze-thaw protocols; however, the best to date is slow cooling to -40 degrees C and rapid thawing from -196 degrees C. If one carefully follows the three distinct steps of freezing and thawing human islets can be successfully cryopreserved, allowing for the establishment of a low temperature bank with diverse HLA (Human Leukocyte Antigen) and ABO phenotypes. Using a combination of fresh and cryopreserved islets to transplant type I diabetic patients, long-term insulin independence has been obtained.

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AnimalsCryopreservationHumansIslets of LangerhansJournal ArticleResearch Support, Non-U.S. Gov'tReview

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BibTeXRIS

R V Rajotte. 1999-06-18. Islet cryopreservation protocols.. https://doi.org/10.1111/j.1749-6632.1999.tb08504.x

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