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PubMed · 10419650

Ingested interferon alpha induces Mx mRNA.

S A Brod·L Nelson·R Jin·J S Wolinsky
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Abstract

We have demonstrated that ingested murine interferon alpha (IFN-alpha) suppressed clinical relapse in chronic relapsing experimental autoimmune encephalomyelitis (CR-EAE), decreased inflammation and suppressed the adoptive transfer of EAE, and is a biological response modifier in patients with multiple sclerosis. We examined the relative levels of the Mx mRNA signal using semiquantitative reverse transcription-polymerase chain reaction analysis on splenocytes from mice and peripheral blood mononuclear cells from man after IFN-alpha ingestion. Both mice and man demonstrated inducible levels of Mx mRNA after ingesting IFN-alpha. Murine spleen T cells and CD8(+)T cells also demonstrated upregulation of Mx mRNA. Murine whole splenocytes demonstrated upregulation of Mx mRNA after IFN-alpha ingestion of 10 and 100 U, but not after 0, 1000, 5000 U. Ingested IFN-alpha acts via established pathways of type 1 IFN signalling.

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Administration, OralAnimalsAntiviral AgentsDensitometryDose-Response Relationship, DrugFemaleGTP-Binding ProteinsHumansImmunologic FactorsInterferon-alphaMaleMiceMyxovirus Resistance ProteinsProteinsRNA, MessengerSpleenJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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S A Brod, L Nelson, R Jin, J S Wolinsky. 1999. Ingested interferon alpha induces Mx mRNA.. https://doi.org/10.1006/cyto.1998.0450

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