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Detecting protein function and protein-protein interactions from genome sequences.

E M Marcotte·M Pellegrini·H L Ng·D W Rice·T O Yeates·D Eisenberg
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Abstract

A computational method is proposed for inferring protein interactions from genome sequences on the basis of the observation that some pairs of interacting proteins have homologs in another organism fused into a single protein chain. Searching sequences from many genomes revealed 6809 such putative protein-protein interactions in Escherichia coli and 45,502 in yeast. Many members of these pairs were confirmed as functionally related; computational filtering further enriches for interactions. Some proteins have links to several other proteins; these coupled links appear to represent functional interactions such as complexes or pathways. Experimentally confirmed interacting pairs are documented in a Database of Interacting Proteins.

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Amino Acid SequenceBacterial ProteinsBinding SitesComputational BiologyDatabases, FactualEscherichia coliEvolution, MolecularFungal ProteinsGenomeGenome, BacterialGenome, FungalHumansModels, BiologicalProteinsSequence Homology, Amino AcidSequence Homology, Nucleic AcidThermodynamicsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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E M Marcotte, M Pellegrini, H L Ng, D W Rice, T O Yeates, D Eisenberg. 1999-07-30. Detecting protein function and protein-protein interactions from genome sequences.. https://doi.org/10.1126/science.285.5428.751

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