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Cortexillin I is required for development in Polysphondylium.

P Fey·E C Cox
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Abstract

The actin binding proteins cortexillin I and II play a major role in Dictyostelium cytokinesis, in which they are found localized to the membranes of the cleavage furrow. Here we report on cortexillin I mutants isolated by gene trapping in Polysphondylium. The original mutation and reconstructed versions of the original, as well as cortexillin I deletions, are unable to form aggregation streams under starvation conditions. The fruiting bodies that do form when cells are grown on bacterial lawns lack the one- and two-dimensional symmetries so apparent in wild type. These two phenotypes and the proposed structural basis for them suggest that cortexillin I functions in chemotaxis and morphogenesis in addition to its role in cytokinesis.

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Amino Acid SequenceAnimalsBridged Bicyclo Compounds, HeterocyclicCell DivisionChemotaxisCloning, MolecularCytoskeletonDimerizationGene DosageGene Expression Regulation, DevelopmentalMicrofilament ProteinsMolecular Sequence DataMorphogenesisMutationMyxomycetesPolymerase Chain ReactionProtein IsoformsProtozoan ProteinsSequence Homology, Amino AcidThiazolesThiazolidinesComparative StudyJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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P Fey, E C Cox. 1999-08-15. Cortexillin I is required for development in Polysphondylium.. https://doi.org/10.1006/dbio.1999.9353

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