A novel quantitative multiplex NASBA method: application to measuring tissue factor and CD14 mRNA levels in human monocytes.

P B van Deursen; A W Gunther; C C van Riel; M M van der Eijnden; H L Vos; B van Gemen; D A van Strijp; N M Tackent; R M Bertina

doi:10.1093/nar/27.17.e15

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A novel quantitative multiplex NASBA method: application to measuring tissue factor and CD14 mRNA levels in human monocytes.

P B van Deursen·A W Gunther·C C van Riel·M M van der Eijnden·H L Vos·B van Gemen·D A van Strijp·N M Tackent·R M Bertina

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Abstract

A new method to quantify two individual mRNAs in a single NASBA reaction is described. In this study, tissue factor and CD14 mRNAs were used as a model system. RNA ratios of -4 to +4 log units were determined with good precision (within 0.3 log) and accuracy (within 0.2 log). By measuring both mRNAs in human monocytes that were stimulated with LPS, the multiplex Q-NASBA proved to be a successful tool to monitor the expression levels of two individual mRNAs in a single-tube amplification system. The method has potential in all fields in which quantitative information is needed on two individual RNAs.

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Biological Assay Cells, Cultured Genetic Markers Humans Lipopolysaccharide Receptors Lipopolysaccharides Monocytes Nucleic Acid Amplification Techniques RNA, Messenger Reproducibility of Results Thromboplastin Time Factors Journal Article Research Support, Non-U.S. Gov't

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P B van Deursen, A W Gunther, C C van Riel, M M van der Eijnden, H L Vos, B van Gemen, D A van Strijp, N M Tackent, R M Bertina. 1999-09-01. A novel quantitative multiplex NASBA method: application to measuring tissue factor and CD14 mRNA levels in human monocytes.. https://doi.org/10.1093/nar%2F27.17.e15

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