High efficiency gene transfer by multiple transfection protocol.

M Yamamoto; S Okumura; C Schwencke; J Sadoshima; Y Ishikawa

doi:10.1023/a:1003598614323

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High efficiency gene transfer by multiple transfection protocol.

M Yamamoto·S Okumura·C Schwencke·J Sadoshima·Y Ishikawa

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Abstract

A high efficiency transfection protocol employing a common polycationic lipid is described. Using LipofectAMINE, a widely used transfection reagent, we transfected 293T cells with a plasmid harboring the beta-galactosidase (beta-gal) gene. The transfection efficiency was determined by direct staining for X-gal. The conventional transfection protocol achieved an efficiency of <40% while our protocol, which employs the repetition of transfection a few times, achieved an efficiency of approximately 80%. Thus, a dramatic increase in transfection efficiency can be obtained by simply repeating transfection with the use of a common polycationic lipid. This method will be useful in many molecular biological experiments.

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Animals COS Cells Cation Exchange Resins Cells, Cultured Drug Carriers Gene Transfer Techniques Lipids Transfection beta-Galactosidase Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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M Yamamoto, S Okumura, C Schwencke, J Sadoshima, Y Ishikawa. 1999. High efficiency gene transfer by multiple transfection protocol.. https://doi.org/10.1023/a%3A1003598614323

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