Oligonucleotide dendrimers: stable nano-structures.

M S Shchepinov; K U Mir; J K Elder; M D Frank-Kamenetskii; E M Southern

doi:10.1093/nar/27.15.3035

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PubMed · 10454597

Oligonucleotide dendrimers: stable nano-structures.

M S Shchepinov·K U Mir·J K Elder·M D Frank-Kamenetskii·E M Southern

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Abstract

DNA dendrimers with two, three, six, nine or 27 arms were reassociated as complementary pairs in solution or with an array of complementary oligonucleotides on a solid support. In all cases, duplex stabilities were greater than those of unbranched molecules of equal length. A theoretical treatment for the process of dissociation of dendrimers explains the major properties of the complexes. The favourable features of DNA dendrimers-their enhanced stability and the simple predictability of their association behaviour-makes them promising as building blocks for the 'bottom up' approach to nano-assembly. These features also suggest applications in oligonucleotide array/DNA chip technology when higher hybridisation temperatures are required, for example, to melt secon-dary structure in the target.

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Base Sequence Drug Design Kinetics Models, Molecular Models, Theoretical Molecular Weight Nucleic Acid Conformation Nucleic Acid Hybridization Oligodeoxyribonucleotides Temperature Thermodynamics Journal Article

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M S Shchepinov, K U Mir, J K Elder, M D Frank-Kamenetskii, E M Southern. 1999-08-01. Oligonucleotide dendrimers: stable nano-structures.. https://doi.org/10.1093/nar%2F27.15.3035

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