[Angiogenesis in vitro].

J Hayashi

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PubMed · 10457903

[Angiogenesis in vitro].

J Hayashi

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Abstract

A quantitative angiogenesis in vitro was investigated by culturing bovine carotid artery endothelial cells between two layers of type I collagen gel. Cells become organized into tube-like structures within few days. Ultrastructurally, tubular structures were composed of one to several endothelial cells containing pinocytotic vesicles and cytoplasmic projections, and linked by junctional complexes. A basal lamina-like structure surrounded the abluminal surface. Glucose, insulin, insulin-like growth factor I at pathophysiological high concentrations significantly stimulated tube-forming activity of endothelial cells by stimulating cell migration.

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Animals Cattle Cell Culture Techniques Cell Movement Cells, Cultured Collagen Dose-Response Relationship, Drug Endothelium, Vascular Glucose Insulin Insulin-Like Growth Factor I Neovascularization, Physiologic Stimulation, Chemical English Abstract Journal Article

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J Hayashi. 1999. [Angiogenesis in vitro].. https://pubmed.ncbi.nlm.nih.gov/10457903/

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