A simple method for constructing E1- and E1/E4-deleted recombinant adenoviral vectors.

H Mizuguchi; M A Kay

doi:10.1089/10430349950017374

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PubMed · 10466635

A simple method for constructing E1- and E1/E4-deleted recombinant adenoviral vectors.

H Mizuguchi·M A Kay

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Abstract

We previously developed a two-plasmid in vitro ligation method that did not require a recombination step to produce new recombinant E1- or E1/E3-deleted adenoviral vectors. In this study, we have modified the system to improve the simplicity of vector construction and, in addition, to allow for production of an E1/E4-deleted vector.

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Adenoviridae Adenovirus E1 Proteins Adenovirus E3 Proteins Adenovirus E4 Proteins Gene Deletion Genetic Vectors Plasmids Recombinant Proteins beta-Galactosidase Journal Article Research Support, U.S. Gov't, P.H.S.

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H Mizuguchi, M A Kay. 1999-08-10. A simple method for constructing E1- and E1/E4-deleted recombinant adenoviral vectors.. https://doi.org/10.1089/10430349950017374

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