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Severe injury triggers antigen-specific T-helper cell dysfunction.

J L Kelly·C B O'Suilleabhain·C C Soberg·J A Mannick·J A Lederer
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Abstract

Although it is established that post-injury immune dysfunction involves alterations in T-cell function, the effects of injury on T-cell function in vivo are poorly understood. This study uses a mouse injury model and an antigen immunization approach to investigate the influence of injury on antigen-specific T-helper cell function. We report here that injury triggered a significant reduction in antigen-specific T-helper-1 (Th1)-dependent IgG2a antibody formation, while IgM, IgG1, and IgE production was unchanged. In addition, injury caused a reduction in cytokine production (IL-2, IFNgamma and IL-10) by antigen-stimulated T-cells. We also demonstrate that interleukin 12 (IL-12), a cytokine that promotes Th1 cell differentiation, restored IgG2a antibody formation and corrected the injury-induced reduction in antigen-stimulated cytokine production. Taken together, these findings indicate that severe injury induces a dramatic reduction in Th1 cell function in vivo and suggest that therapies designed to restore Th1 cell function may be beneficial to the injured host.

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AnimalsAntibody FormationAntigen PresentationBurnsImmunityImmunizationInterferon-gammaInterleukin-10Interleukin-2MaleMiceTh1 CellsTh2 CellsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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J L Kelly, C B O'Suilleabhain, C C Soberg, J A Mannick, J A Lederer. 1999. Severe injury triggers antigen-specific T-helper cell dysfunction.. https://doi.org/10.1097/00024382-199907000-00006

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