Chip-based genotyping by mass spectrometry.

K Tang; D J Fu; D Julien; A Braun; C R Cantor; H Köster

doi:10.1073/pnas.96.18.10016

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PubMed · 10468554

Chip-based genotyping by mass spectrometry.

K Tang·D J Fu·D Julien·A Braun·C R Cantor·H Köster

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Abstract

Silicon chips with immobilized target DNAs were used for accurate genotyping by mass spectrometry. Genomic DNAs were amplified with PCR, and the amplified products were covalently attached to chip wells via N-succinimidyl (4-iodoacetyl)aminobenzoate (SIAB) chemistry. Primer annealing, extension, and termination were performed on a 1-microl scale directly in the chip wells in parallel. Diagnostic products thus generated were detected in situ by using matrix-assisted laser desorption ionization mass spectrometry. This miniaturized method has the potential for accurate, high-throughput, low-cost identification of genetic variations.

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Base Sequence Cross-Linking Reagents DNA DNA Primers Genotype Microscopy, Electron, Scanning Polymerase Chain Reaction Silicon Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Succinimides Journal Article

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K Tang, D J Fu, D Julien, A Braun, C R Cantor, H Köster. 1999-08-31. Chip-based genotyping by mass spectrometry.. https://doi.org/10.1073/pnas.96.18.10016

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