Antimicrobial susceptibility testing of Chlamydia trachomatis using a reverse transcriptase PCR-based method.

N A Cross; D J Kellock; G R Kinghorn; M Taraktchoglou; E Bataki; K M Oxley; P M Hawkey; A Eley

doi:10.1128/aac.43.9.2311

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Antimicrobial susceptibility testing of Chlamydia trachomatis using a reverse transcriptase PCR-based method.

N A Cross·D J Kellock·G R Kinghorn·M Taraktchoglou·E Bataki·K M Oxley·P M Hawkey·A Eley

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Abstract

The conventional method for antimicrobial susceptibility testing of Chlamydia trachomatis is subjective and potentially misleading. We have developed a reverse transcriptase PCR (RT-PCR)-based method which is more sensitive and less subjective than the conventional method. Using 16 strains of C. trachomatis in triplicate assays, we found the RT-PCR method consistently more sensitive than the conventional technique for all eight antimicrobials tested, with resultant MICs determined by RT-PCR ranging from 1.6-fold higher (erythromycin) to >/=195-fold higher (amoxicillin).

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Amoxicillin Anti-Bacterial Agents Chlamydia trachomatis Erythromycin Female Humans Microbial Sensitivity Tests Penicillins Reverse Transcriptase Polymerase Chain Reaction Sensitivity and Specificity Comparative Study Journal Article Research Support, Non-U.S. Gov't

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N A Cross, D J Kellock, G R Kinghorn, M Taraktchoglou, E Bataki, K M Oxley, P M Hawkey, A Eley. 1999. Antimicrobial susceptibility testing of Chlamydia trachomatis using a reverse transcriptase PCR-based method.. https://doi.org/10.1128/aac.43.9.2311

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