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PubMed · 10479555

Experimental aspects of multidimensional solid-state NMR correlation spectroscopy.

A Ramamoorthy·C H Wu·S J Opella
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Abstract

The experimental parameters critical for the implementation of multidimensional solid-state NMR experiments that incorporate heteronuclear spin exchange at the magic angle are discussed. This family of experiments is exemplified by the three-dimensional experiment that correlates the (1)H chemical shift, (1)H-(15)N dipolar coupling, and (15)N chemical shift frequencies. The broadening effects of the homonuclear (1)H-(1)H dipolar couplings are suppressed using flip-flop (phase- and frequency-switched) Lee-Goldburg irradiations in both the (1)H chemical shift and the (1)H-(15)N dipolar coupling dimensions. The experiments are illustrated using the (1)H and (15)N chemical shift and dipolar couplings in a single crystal of (15)N-acetylleucine.

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Hydrogen BondingLeucineNitrogen IsotopesNuclear Magnetic Resonance, BiomolecularJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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A Ramamoorthy, C H Wu, S J Opella. 1999. Experimental aspects of multidimensional solid-state NMR correlation spectroscopy.. https://doi.org/10.1006/jmre.1999.1827

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