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PubMed · 10481977

Empirical relationship between chiral selectivity and mobile phase modifier properties.

J A Blackwell·R W Stringham·D Xiang·R E Waltermire
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Abstract

An empirical relationship was derived which relates properties of the mobile phase modifier to the chiral selectivity factor for a given analyte/chiral selector combination. Using carbon dioxide and heptane-based mobile phases, the effect of various mobile phase modifiers on Pirkle-type stationary phases may be accurately modeled using a two-parameter equation. Similar results are obtained using cellulosic stationary phases with carbon dioxide-based mobile phases. Modeling separations performed using heptane-based mobile phases with cellulosic stationary phases were not successful. The predictive ability of this modeling approach was demonstrated using novel modifiers and chiral analytes.

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Chromatography, LiquidSolventsSpectrophotometry, UltravioletStereoisomerismJournal Article

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J A Blackwell, R W Stringham, D Xiang, R E Waltermire. 1999-08-13. Empirical relationship between chiral selectivity and mobile phase modifier properties.. https://doi.org/10.1016/s0021-9673(99)00637-8

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