High throughput fluorescent CE-SSCP SNP genotyping.

D Gonen; J Veenstra-VanderWeele; Z Yang; B Leventhal; E H Cook

doi:10.1038/sj.mp.4000564

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High throughput fluorescent CE-SSCP SNP genotyping.

D Gonen·J Veenstra-VanderWeele·Z Yang·B Leventhal·E H Cook

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Abstract

Large numbers of single nucleotide polymorphisms (SNPs) are being identified by several laboratories for the purpose of developing dense genetic maps. Single-strand conformation polymorphism (SSCP) analysis has been widely used as a method for detecting novel sequence variations in PCR products. Differences in migration of single-stranded DNA can be used not only to find mutations, but to genotype SNPs in large sample populations. Using PCR with fluorescent labeling and automated capillary electrophoresis SSCP (CE-SSCP), we have developed a panel of 15 functional candidate SNPs. With an automated single capillary instrument, relatively rapid and low cost CE-SSCP SNP genotyping using currently available technology is feasible for 135 000 genotypes per year. With parallel multiple array capillary electrophoresis, more genotypes per year may be attainable.

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Automation Chromosome Mapping DNA Primers Electrophoresis, Capillary Fluorescent Dyes Genotype Humans Point Mutation Polymerase Chain Reaction Polymorphism, Genetic Polymorphism, Single-Stranded Conformational Proteins Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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D Gonen, J Veenstra-VanderWeele, Z Yang, B Leventhal, E H Cook. 1999. High throughput fluorescent CE-SSCP SNP genotyping.. https://doi.org/10.1038/sj.mp.4000564

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